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Enzymatic Production of Ferulic Acid from Defatted Rice Bran by Using a Combination of Bacterial Enzymes |
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| Authors: | Misugi Uraji Masayo Kimura Yosikazu Inoue Kayoko Kawakami Yuya Kumagai Koichi Harazono Tadashi Hatanaka |
| Institution: | 1. Okayama Prefectural Technology Center for Agriculture, Forestry, and Fisheries, Research Institute for Biological Sciences (RIBS), 7549-1 Kibichuo-cho, Kaga-gun, Okayama, 716-1241, Japan 2. Satake Corporation, 2-30 Saijo Nishihonmachi, Higashi-Hiroshima-shi, Hiroshima, 739-8602, Japan 3. Research & Development Center, Nagase & Company Limited, 2-3 Murotani, 2-chome, Nishi-ku, Kobe, Hyogo, 651-2241, Japan |
| Abstract: | Ferulic acid (FA), which is present in the cell walls of some plants, is best known for its antioxidant property. By combining a commercial enzyme that shows FA esterase activity with several Streptomyces carbohydrate-hydrolyzing enzymes, we succeeded in enhancing the enzymatic production of FA from defatted rice bran. In particular, the combination of three xylanases, an α-l-arabinofuranosidase, and an acetyl xylan esterase from Streptomyces spp. produced the highest increase in the amount of released FAs among all the enzymes in the Streptomyces enzymes library. This enzyme combination also had an effect on FA production from other biomasses, such as raw rice bran, wheat bran, and corncob. |
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