| 台湾眼镜蛇毒的NMR研究 |
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| 引用本文： | 余靖.台湾眼镜蛇毒的NMR研究[J].波谱学杂志,1995,12(4):357-372. |
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| 作者姓名： | 余靖 |
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| 作者单位： | 清华大学化学系, 新竹台湾 |
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| 摘 要： | 随着核磁共振技术的不断创新及分子模拟计算的长足进步,用NMR结合电脑计算已成为研究蛋白质结构及功能的主要方法,本文以实际的范例,介绍台湾眼镜蛇毒的NMR研究成果,台湾NMR的研究工作正在蓬勃发展,目前最高磁场为600MHz,分别装置在中研院生物医学研究所(台北)及清华大学化学系(新竹)。
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| 关 键 词： | 神经毒蛋白 心脏毒蛋白 β-折片 α-螺旋 质子间距 距离几何软件 淬冷回温 主键导向 序列特定判定 秩序参数 |
THE NMR RESEARCH ON TAIWAN COBRA |
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| Chin Yu.THE NMR RESEARCH ON TAIWAN COBRA[J].Chinese Journal of Magnetic Resonance,1995,12(4):357-372. |
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| Authors: | Chin Yu |
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| Institution: | Dcpartment of Chemistry National Tsing Hua University Hsinchu, Taiwan |
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| Abstract: | The recent researeh on the snake venom proteins in Taiwan is described.The solution structures of Cobrotoxin and Cardiotoxin from Taiwan cobra (Naja naja atra) were deteimned using 2D NMR and dynamical siniulated annealing methods.NMR experiments such as DQF-COSY,TOCSY and NOESY were used to complete the full assignments by sequcnce-specific and main-chain-directed methods.Constraints derived from NMR data were used during the distance-gcometry calculation and refinement stage in X-PLOR.Comparison of their structures indicates that they process the similar backbone conformations with the emergence of three independent loops from the central core (formed by four disulfides) of the inolecules. |
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| Keywords: | Neurotoxin Cardintoxin β-sheet α-helix Interproton distance Distance geometry algorithm Simulated annealing Main-chain-dasected Sequcnce specific assignment Order parameter |
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