| 稀土离子对人血清白蛋白动态结构性质影响的23Na NMR研究 |
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| 引用本文： | 任吉民,张善荣.稀土离子对人血清白蛋白动态结构性质影响的23Na NMR研究[J].波谱学杂志,1995,12(3):253-259. |
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| 作者姓名： | 任吉民 张善荣 |
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| 作者单位： | 中国科学院长春应用化学研究所应用谱学开放实验室, 长春 130022 |
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| 基金项目： | 中国科学院长春应用化学研究所应用谱学开放实验室资助 |
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| 摘 要： | 利用23Na作为探针磁核,通过弛豫分析发现:稀土离子与HSA络合后使蛋白质分子体积膨胀,链段活动性增加,表现为相关时间(τc)减小;HSA可能至少有了3个高亲和位点可络合稀土离子;稀土离子诱导的HSA动态结构变化在某种程度上具有可逆性,即当高亲和位上的稀土离子被螯合剂在取后,膨胀伸展的结构趋于恢复原有状态。
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| 关 键 词： | 人血清白蛋白 稀土离子 23Na NMR 弛豫分析 |
| 收稿时间： | 1994-09-12 |
THE EFFECTS OF RARE EARTH IONS ON THE DYNAMIC STRUCTURE OF HSA.A 23Na NMR STUDY |
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| Ren Jimin,Zhang Shanrong,Liu Aizhuo,Pei Fengkui.THE EFFECTS OF RARE EARTH IONS ON THE DYNAMIC STRUCTURE OF HSA.A 23Na NMR STUDY[J].Chinese Journal of Magnetic Resonance,1995,12(3):253-259. |
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| Authors: | Ren Jimin Zhang Shanrong Liu Aizhuo Pei Fengkui |
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| Institution: | Applied Spectroscopy Lab, Changchun Institute of Applied Chemistry, The Chinese Academy of Sciences, Changchun 130022 |
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| Abstract: | The effects of rare earth ions (Y3+,La3+ and Lu3+) on the dynamic structure of HSA were studied by 23Na NMR spectroscopy.The protein would be expanded and the mobilities of the molecular backbone would be enlarged by binding the rare earth ions,which would lead to the decrease of the correlation time (τc).There are three or more highly rare earth binding sites on HSA.The dynamic structural change is revelsible to some degrees,that is,the original state would be recovered if the highly binding rare earth ions were removed by chelating agents. |
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| Keywords: | HSA Rare earth ions 23Na NMR Relaxation analysis |
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