Counter-current chromatography separation scaled up from an analytical column to a production column |
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Authors: | Wood Philip Ignatova Svetlana Janaway Lee Keay David Hawes David Garrard Ian Sutherland Ian A |
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Institution: | Brunel Institute for Bioengineering, Brunel University, Uxbridge UB8 3PH, UK. philip.wood@brunel.ac.uk |
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Abstract: | An analytical separation was performed on an analytical J-type counter-current chromatography (CCC) instrument using a 5.4 ml column, with a 1 ml/min mobile phase flow rate. This separation had a resolution of 0.69 and was achieved in 10 min. The same separation was performed using two 2300 ml columns connected in series at a flow rate of 850 ml/min using a production scale J-type centrifuge. This production scale separation was also obtained in 10 min with a resolution of 0.71. This represents an 850 times increase in productivity. This paper presents these separations and the underlying scale up theory. |
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