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Micro-flow injection system for the urinary protein assay |
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| Authors: | Nishihama Syouhei Imabayashi Hisano Matoba Tomoko Toya Chika Watanabe Kosuke Yoshizuka Kazuharu |
| Institution: | aDepartment of Chemical Engineering, Faculty of Environmental Engineering, The University of Kitakyushu, Hibikino 1-1, Kitakyushu 808-0135, Japan bKitakyushu Foundation for the Advancement of Industry, Science and Technology, Hibikino 2-1, Kitakyushu 808-0135, Japan |
| Abstract: | A urinary protein assay has been investigated, employing a micro-flow injection analysis (μFIA) combined with an adsorptive separation of protein from analyte. The adsorptive separation part of protein in the artificial urine with ceramic hydroxyapatite is integrated on the μFIA chip, since the interference of other components coexisting in urine occurs in the conventional FIA system. The typical FI peak can be obtained following the adsorption–elution process of the protein prior to the detection, and the protein concentration in artificial urine can be quantitatively determined. |
| Keywords: | μTAS Micro-flow injection analysis Urinary protein assay |
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