| 重氮法固定化糖化酶活力和稳定性 |
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| 引用本文： | 孔维,周慧.重氮法固定化糖化酶活力和稳定性[J].高等学校化学学报,1994,15(5):681-684. |
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| 作者姓名： | 孔维 周慧 |
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| 作者单位： | 吉林大学分子生物学系 |
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| 摘 要： | 本文以苯氨基磺酸型多孔聚苯乙烯为载体，重氮化活化其苯氨基与糖化酶上的氨基反应。在固定化酶过程中，随反应时间的延长，不但固定化酶总活力增加，而且其比活和热稳定性也随之升高，最终可得活力为２００００Ｕ／ｇ干胶，比活为５００的高活力固定化酶，该固定化酶在ｐＨ４．５，６０℃无底物下保温，其半寿期是天然酶的５倍。以麦芽糖，糊精（分子量为１５００）和可溶性淀粉为底物测定该固定化酶活力表明：对某种孔度一定的载体
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| 关 键 词： | 固定化酶 糖化酶 重氮法 酶制剂 |
The Activity and Thermostability of Immobilized Glucoamylase by Diazotization |
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| KONG Wei,ZHOU Hui,WANG Li-Ping,CHEN Zun,Li Wei,SHEN Jia-Cong.The Activity and Thermostability of Immobilized Glucoamylase by Diazotization[J].Chemical Research In Chinese Universities,1994,15(5):681-684. |
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| Authors: | KONG Wei ZHOU Hui WANG Li-Ping CHEN Zun Li Wei SHEN Jia-Cong |
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| Abstract: | A method getting high activity and thermostability for the immobilized glucoamylase was found out.The glucoamylase was immobilized on porous anilin0-sulfonic polystyrene beads by diazotization.The effects of immobilizing pH,immobilizing time and molecular weight of the substrate on the activity and thermostability of immobilized glucoamylase were investigated.The results obtained show that by prolonging the coupling time of diazol-protein,the activity of immobilized glucoamylase got to 20 000 mg/hour g dry gel,and the thermostability was 5 times higlier than that of soluble glucoamylase. |
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| Keywords: | Porous ionic carrier Immobilized enzyme Glucoamylase |
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