Benzoisothiazole-1,1-dioxide-based synthetic receptor for zinc ion recognition in aqueous medium and its interaction with nucleic acids |
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| Authors: | Milan Jakubek Zdeněk Kejík Veronika Antonyová Robert Kaplánek David Sýkora Róbert Hromádka |
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| Affiliation: | 1. Department of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, Prague, Czech Republic;2. BIOCEV, First Faculty of Medicine, Charles University, Czech Republic;3. Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague Ke Karlovu, Prague Czech Republic;4. BIOCEV, First Faculty of Medicine, Charles University, Czech Republic;5. C2P s.r.o. Jungmannova 101, 503 51 Chlumec nad Cidlinou, Czech Republic |
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| Abstract: | Benzoisothiazole-1,1-dioxide-based synthetic receptor was prepared by two step synthesis in 92 % overall yield. Its applicability for the determination of Zn(II) and interction with nucleic acids was studied by absorption spectroscopy. Obtained data, specifically low limit of detection, 0.15 µM (R2 = 0.9933), showed the high potential of the tested structure motif for the recognition and determination of Zn(II) ions in aqueous media (water:DMSO; 99:1 (ν/ν)). Alone receptor displayed orderly strongly RNA affinity. Value of LogK was 6.1 and 4.9 for its complex (1:1) with RNA and DNA, respectively. Nevertheless, in presence of complexed Zn(II) ions, its DNA affinity (represent by K, LogK = 5.7) strongly grow to near value obtained for its interaction with RNA. On the other hand, its RNA affinity (LogK = 5.9) displayed not significantly change in the presence of complexed one. |
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| Keywords: | Chelator hydrazone nucleic acid zinc ions |
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