Synthesis and properties of molecular imprints of darifenacin: The potential of molecular imprinting for bioanalysis |
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Authors: | R F Venn R J Goody |
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Institution: | (1) Department of Drug Metabolism, Pfizer Central Research, Ramsgate Road, CT13 9NJ Sandwich, Kent, UK |
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Abstract: | Summary A molecularly imprinted polymer has been developed which subsequently demonstrated an ability to selectively retain darifenacin
(UK-88, 525-S) from aqueous acetonitrile when used as a stationary phase in HPLC columns and as a packing in solid-phase extraction
cartridges.
The imprinted polymer is applicable to a wide range of analytical methods including extraction from plasma, purification of
radiolabelled UK-88,525, chiral separations and separation of metabolites and structural analogues. The polymer is able to
extract darifenacin directly from a protein-precipitated human plasma/acetonitrile (1:1 v/v) mixture with 100% recovery. The
imprinted polymer can also effect a repurification of14C-labelled darifenacin.
The drawbacks of molecular imprints for ultra-trace bioanalysis (in the sub-nanogram/mL range) are discussed. These centre
on the difficulty of removing all the template from the polymer and the consequent effects of template bleed on assay precision
and accuracy when used as solid-phase extraction cartridges. Possible solutions to this problem are considered.
Presented at: Affinity Chromatography Conference, Cambridge, UK, July 1–3, 1997. |
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Keywords: | Column liquid chromatography Solid-phase extraction Molecular imprinting Bioanalysis |
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