Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix |
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Authors: | Dräger Gerald Kiss Csilla Kunz Ulrich Kirschning Andreas |
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Affiliation: | Institut für Organische Chemie and Zentrum für Biomolekulare Wirkstoffchemie (BMWZ), Leibniz Universit?t Hannover, Schneiderberg 1b, Hannover, Germany. draeger@oci.uni-hannover.de |
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Abstract: | The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly(vinyl benzyl chloride)/poly(vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His(6)-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6), (R)-2-hydroxy-1-phenylpropan-1-one (7) and 6-O-acetyl-D-glucal (17) in a flow-through mode. |
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