Influence of salt ions on binding to molecularly imprinted polymers |
| |
Authors: | Henrik Kempe Maria Kempe |
| |
Affiliation: | (1) Biomedical Polymer Technology, Department of Experimental Medical Science, Biomedical Center, D11, Lund University, 221 84 Lund, Sweden; |
| |
Abstract: | Salt ions were found to have an influence on template binding to two model molecularly imprinted polymers (MIPs), targeted to penicillin G and propranolol, respectively, in water–acetonitrile mixtures. Water was detrimental to rebinding of penicillin G whereas propranolol bound in the entire water–acetonitrile range tested. In 100% aqueous solution, 3-M salt solutions augmented the binding of both templates. The effects followed the Hofmeister series with kosmotropic ions promoting the largest increase. Binding was mainly of a non-specific nature under these conditions. In acetonitrile containing low amounts of water, the specific binding to the MIPs increased with the addition of salts. Binding of penicillin G followed the Hofmeister series while an ion-exchange mechanism was observed for propranolol. The results suggest that hydration of kosmotropic ions reduces the water activity in water-poor media providing a stabilizing effect on water-sensitive MIP–template interactions. The effects were utilized to develop a procedure for molecularly imprinted solid-phase extraction (MISPE) of penicillin G from milk with a recovery of 87%. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|