Immunosensor application based on Raman spectroscopy of porous silicon |
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| Authors: | Tao Jiang Jiaqing Mo Xiaoyi L Pengfei Yan Zhenhong Jia Jiangwei Li and Fuchun Zhang |
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| Institution: | 1School of Physics, Xinjiang University, Urumqi 830046, China 2School of Electronic and Information Engineering, Xi’an Jiaotong University, Xi’an 710049, China 3Key Laboratory of Xinjiang Biological Resources and Gene Engineering, College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China 4College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China |
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| Abstract: | Porous silicon (PS) suitable for optical detection of immunoreaction is fabricated. The structure of immunosensor is prepared by the following steps: oxidization, silanization, glutaraldehyde cross-linker, and covalent binding of antibody. When antigen is added into the immunosensor, the Raman intensity is estimated to be linearly reduced according to the concentration of the surface protective antigen protein A (spaA) of below 4.0 μg ml-1. The ultimate detection limit is 1.412 × 102 pg ml-1. Controlled experiments are also presented with non-immune antigen of the spaA, and results show that the immunosensor has high specificity. Compared with the conventional enzyme-linked immuno sorbent assay (ELISA), this method is quick, inexpensive, and label-free. |
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