A three-way array model for resolving multicomponent fluorescent mixtures |
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Authors: | Xinming Tu Donald S Burdick |
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Institution: | (1) Harvard University School of Public of Health, 02115 Boston, MA, U.S.A.;(2) Duke University, 27706 Durham, NC, U.S.A. |
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Abstract: | Fluorescence lifetime provides a third independent dimension of information for the resolution of total luminescence spectra of multicomponent mixtures. The incorporation of this parameter into the Excitation-Emission Matrix (EEM) by the phase-modulation technique results in a three-dimensional Excitation-Emission-Frequency Array (EEFA). Multicomponent analysis based on the three-dimensional EEFA brings a qualitative change for the resolved spectra, i.e., individual spectra can be uniquely resolved, which is impossible with any two-dimensional analysis. In this paper, we present a method for analyzing the EEFA. We show mathematically that with the three-dimensional analysis of the EEFA individual spectra and lifetimes can be obtained. Our algorithm is developed in mathematical detail and demonstrated by its application to a two-component mixture. |
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