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DNA Binding Mode of Antibody Fragments Specific for TT Photo-Dimers |
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| Authors: | Hiroyuki Kobayashi Yasuo Komatsu Hiroshi Morioka Eiko Ohtsuka |
| Institution: | Graduate School of Pharmaceutical Sciences , Hokkaido University , Sapporo , 060-0812 , Japan |
| Abstract: | A single-chain antibody specific for the pyrimidine(6-4)pyrimidone photoproduct in DNA was constructed and its binding properties to the cognate oligonucleotides were investigated. The fluorescent property of 2-aminopurine was used to study the binding mode of the antibody fragment to the pyrimidine(6-4)pyrimidone photoproduct in double-stranded DNA. The results indicated that the single-chain antibody recognizes the lesion in the single-stranded state. |
| Keywords: | Antibodies Fluorescence Oligodeoxyribonucleotide Pyrimidine(6-4)pyrimidone Photoproduct Surface Plasmon Resonance |