1. Department of Chemistry, University of Arizona , Tucson, USA;2. Institute of Genetics and Microbiology, University of Munich , Munich, Germany
Abstract:
Abstract Chemical and biochemical methods were used to unravel the unprecedented pathway by which the CN ligands of iron in [NiFe] hydrogenase are introduced. Carbamoyl phosphate is the one carbon precursor of these ligands, and reactions involving a protein cysteinyl sulfur are key for processing this precursor into CN ligands.