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Generation of the [NiFe] Hydrogenase Active Site (Sulfur's the One!) |
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| Authors: | Richard S Glass Athanasios Paschos Stephanie Reissmann M S Singh Haofan Wang August Böck |
| Institution: | 1. Department of Chemistry, University of Arizona , Tucson, USA;2. Institute of Genetics and Microbiology, University of Munich , Munich, Germany |
| Abstract: | Abstract Chemical and biochemical methods were used to unravel the unprecedented pathway by which the CN ligands of iron in NiFe] hydrogenase are introduced. Carbamoyl phosphate is the one carbon precursor of these ligands, and reactions involving a protein cysteinyl sulfur are key for processing this precursor into CN ligands. |
| Keywords: | Carbamoyl phosphate [NiFe] hydrogenase biosynthesis thiocarbamate thiocyanate |