| 邻苯二甲醛衍生高效液相色谱法快速测定血小板中组胺含量 |
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| 引用本文： | 谢大年,陈亚立,郭兆贵. 邻苯二甲醛衍生高效液相色谱法快速测定血小板中组胺含量[J]. 色谱, 1994, 12(1): 65-66 |
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| 作者姓名： | 谢大年 陈亚立 郭兆贵 |
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| 作者单位： | 湖南医科大学临床药理国家培训中心 |
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| 摘 要： | An HPLC method for the determination of histamine(HA)in platelet.based on pre-column derivatiza-tion with o-phthalaldehvde(OPA ).is described.The HA was allowed to react with OPA at -10℃ for30min.HPLC was performed on a reversed-phase column with isocratic elution using sodium acetate buffer inmethanol as eluent system.A fluorescence detection system was used;excitation was set at 350.0nm andemission was read at 445.5nm,A good linear relationship was found in the range of 3.75×10￣(-3)～10. 5nmol.
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| 关 键 词： | 高效液相色谱 快速分析 邻苯二甲醛 血小板. 柱前衍生 组胺 |
A Simple and Fast Determination of Histamine in Platelet by High Performance Liquid Chromatography(HPLC)with Pre-column Derivatization with o-Phthalaldehyde |
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| Xie Danian,Chen Yali,Guo Zhaogui. A Simple and Fast Determination of Histamine in Platelet by High Performance Liquid Chromatography(HPLC)with Pre-column Derivatization with o-Phthalaldehyde[J]. Chinese journal of chromatography, 1994, 12(1): 65-66 |
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| Authors: | Xie Danian Chen Yali Guo Zhaogui |
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| Abstract: | |
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| Keywords: | o-phthalaldehyde high performance liquid chromatography histamine rapid analysis pre-column derivatization platelet |
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