Luminescent Strategies for Label‐Free G‐Quadruplex‐Based Enzyme Activity Sensing |
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| Authors: | Dr Dik‐Lung Ma Zhen‐Zhen Dong Dr Kasipandi Vellaisamy Ka‐Man Cheung Guanjun Yang Dr Chung‐Hang Leung |
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| Institution: | 1. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong;2. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical SciencesUniversity of Macau, Macao |
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| Abstract: | By catalyzing highly specific and tightly controlled chemical reactions, enzymes are essential to maintaining normal cellular physiology. However, aberrant enzymatic activity can be linked to the pathogenesis of various diseases. Therefore, the unusual activity of particular enzymes can represent testable biomarkers for the diagnosis or screening of certain diseases. In recent years, G‐quadruplex‐based platforms have attracted wide attention for the monitoring of enzymatic activities. In this Personal Account, we discuss our group's works on the development of G‐quadruplex‐based sensing system for enzyme activities by using mainly iridium(III) complexes as luminescent label‐free probes. These studies showcase the versatility of the G‐quadruplex for developing assays for a variety of different enzymes. |
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| Keywords: | G-quadruplex enzyme iridium(III) complex luminescent enhancement |
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