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A Method for Measuring the Three-Dimensional Refractive-Index Distribution of Single Cells Using Proximal Two-Beam Optical Tweezers and a Phase-Shifting Mach—Zehnder Interferometer |
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| Authors: | Toshiki Yasokawa Ichirou Ishimaru Masahiro Kondo Shigeki Kuriyama Tsutomu Masaki Kaoru Takegawa Naotaka Tanaka |
| Affiliation: | (1) Faculty of Engineering, Kagawa University, 2217-20, Hayashicho Takamatsu, 761-0396, Japan;(2) Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun Kagawa, 761-0793, Japan;(3) Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun Kagawa, 761-0795, Japan |
| Abstract: | This paper describes a method for measuring the three-dimensional (3D) refractive-index distribution in a single cell. The method can be used to observe the distribution of cell components without fluorescence staining. The two-dimensional optical path length distributions from multiple directions are obtained by non-contact rotation of the cell. These optical path lengths are converted into the line integrals of the refractive index, and the 3D refractive-index distribution is reconstructed by means of computed tomography. The refractive-index distribution in a breast cancer cell can be measured using a phase-shifting Mach—Zehnder interferometer in conjunction with proximal two-beam optical tweezers. |
| Keywords: | cell refractive index three-dimensional imaging computed tomography rotation optical tweezers light pressure phase shift interferometer Mach— Zehnder interferometer |
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