| Sensitive analysis of amino acids in injection liquor by reversed-phase HPLC |
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| 引用本文： | SHANG,Zhen-Hua YU,Yi-Nian GUO,Wei JIANG,Hui ZHOU,Liaug-MoDalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,Liaoning 116012,China. Sensitive analysis of amino acids in injection liquor by reversed-phase HPLC[J]. 中国化学, 1995, 13(2): 163-168. DOI: 10.1002/cjoc.19950130211 |
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| 作者姓名： | SHANG Zhen-Hua YU Yi-Nian GUO Wei JIANG Hui ZHOU Liaug-MoDalian Institute of Chemical Physics Chinese Academy of Sciences Dalian Liaoning 116012 China |
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| 作者单位： | SHANG，Zhen-Hua YU，Yi-Nian GUO，Wei JIANG，Hui ZHOU，Liaug-MoDalian Institute of Chemical Physics，Chinese Academy of Sciences，Dalian，Liaoning 116012，China |
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| 基金项目： | supported by the National Natural Science Foundation of China. |
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| 摘 要： | A convenient derivatization method of amino acids with l-fluoro-2,4-dimtrobenzene as reaction reagent and a separation system were described. The derivative amino acids were separated on a specific chemically bonded phase column with a simple linear gradient elution consisting of aqueous buffer and methanol. The eluate was detected by common ultraviolet absorption detector at 360 nm. The detection limits of amino acids were as low as 10 picomole. This method has been successfully applied to assay amino acid injection liquor used in hospital. It has good repro-ducibility and precision. The procedures avoid the requirements of particular derivative equipment and analyzer employed in conventional amino acid analysis.
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Sensitive analysis of amino acids in injection liquor by reversed-phase HPLC |
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| SHANG,Zhen-Hua YU,Yi-Nian GUO,Wei JIANG,Hui ZHOU,Liaug-MoDalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,Liaoning ,China. Sensitive analysis of amino acids in injection liquor by reversed-phase HPLC[J]. Chinese Journal of Chemistry, 1995, 13(2): 163-168. DOI: 10.1002/cjoc.19950130211 |
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| Authors: | SHANG Zhen-Hua YU Yi-Nian GUO Wei JIANG Hui ZHOU Liaug-MoDalian Institute of Chemical Physics Chinese Academy of Sciences Dalian Liaoning China |
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| Affiliation: | SHANG,Zhen-Hua YU,Yi-Nian GUO,Wei JIANG,Hui ZHOU,Liaug-MoDalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,Liaoning 116012,China |
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| Abstract: | A convenient derivatization method of amino acids with l-fluoro-2,4-dimtrobenzene as reaction reagent and a separation system were described. The derivative amino acids were separated on a specific chemically bonded phase column with a simple linear gradient elution consisting of aqueous buffer and methanol. The eluate was detected by common ultraviolet absorption detector at 360 nm. The detection limits of amino acids were as low as 10 picomole. This method has been successfully applied to assay amino acid injection liquor used in hospital. It has good repro-ducibility and precision. The procedures avoid the requirements of particular derivative equipment and analyzer employed in conventional amino acid analysis. |
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| Keywords: | Amino acids precolumn derivatization reversed phase separation UV absorption determination. |
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