脂质体电动色谱法评价阿魏酸与生物膜的相互作用

利用脂质体与生物膜结构的相似性,将脂质体加入毛细管电泳缓冲溶液中作为假固定相,在数分钟内测定了阿魏酸的脂水分配系数Klw,建立了脂质体电动色谱评价阿魏酸与生物膜相互作用的方法。研究了脂质体中胆固醇的含量、缓冲溶液pH值和缓冲体系对Klw的影响。结果表明,在实验条件范围内(胆固醇含量0~30%,pH值4.0~12.0),胆固醇含量升高,缓冲溶液pH值增大,Klw降低;在不同的缓冲体系中,离子强度越大,Klw越大。脂水分配系数的变化反映了阿魏酸与生物膜相互作用。

Evaluation of Ferulic Acid-Biomembrane Interaction by Liposome Electrokinetic Chromatography

A facile method for evaluating acidic drug-biomembrane interaction was established by liposome electrokinetic chromatography(LEKC).LEKC is a capillary electrophoresis technique where liposome is incorporated into a buffer solution and act as a pseudo-stationary phase using the structure similarity of liposome and biomembrane.Liposome water partition coefficient(Klw) of ferulic acid was determined in several minutes.The effect of cholesterol content,buffer pH and buffer composition on Klw was investigated.An increase in cholesterol content(0-30%)and pH values(4.0-12.0) resulted in a lower Klw.In different buffer systems,the greater ionic strength was,the larger Klw was.The difference of Klw revealed ferulic acid-biomembrane interaction.