Click chemistry to construct fluorescent oligonucleotides for DNA sequencing |
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| Authors: | Seo Tae Seok Li Zengmin Ruparel Hameer Ju Jingyue |
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| Affiliation: | Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. |
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| Abstract: | "Click chemistry" 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with single-base resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection. |
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