Click chemistry to construct fluorescent oligonucleotides for DNA sequencing |
| |
| Authors: | Seo Tae Seok Li Zengmin Ruparel Hameer Ju Jingyue |
| |
| Institution: | Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. |
| |
| Abstract: | "Click chemistry" 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with single-base resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
