循环肿瘤细胞体内检测技术及其应用研究

从实体瘤脱落进入血液循环系统的肿瘤细胞即循环肿瘤细胞(CTCs)与肿瘤转移密切相关,因此CTCs检测对癌症患者的诊断、治疗监测、病情评估以及肿瘤转移机制研究具有重要意义。由于CTCs在体内含量极少、异质性、分布不均一,通过体外采血发展的CTCs检测技术虽然已取得很大进展,但仍然面临肿瘤细胞损失、失活、失真以及灵敏度低等问题,因此亟需发展基于体内快速流动血液的肿瘤细胞检测技术,在真实生理状态下实时监测CTCs动态变化。在此,我们总结了CTCs体内检测技术及其相关应用的研究进展,分析了这些技术的优势和不足。最后,讨论并展望了CTCs体内检测技术的未来发展趋势。

Detection of Circulating Tumor Cell in Vivo:Technology and Application

Cancer cells that shed from solid tumor and circulate into bloodstream, namely circulating tumor cells(CTCs), are closely related to tumor metastasis. Therefore, CTCs detection is of great significance for cancer diagnosis, treatment monitoring, disease assessment and understanding of the mechanisms underlying tumor metastasis. However, CTCs are extraordinarily rare, heterogeneous, nonuniform in blood. Even if strategies for detection of CTCs in static blood collected from patients have made significant progress, they still face the tumor cell loss, cell death, low fidelity, and low sensitivity. It is essential to develop approaches available for detection of tumor cells in fast-flowing blood, allowing real-time monitoring of CTC dynamic changes under physiological conditions. In this review, we summarize techniques developed for CTC detection in vivo and their related applications. Furthermore, the advantages and disadvantages of these techniques are analyzed. Finally, future techniques for detection of CTCs in vivo are discussed and predicted.