锌诱导对香菇菌丝生长速度及金属硫蛋白含量影响

金属硫蛋白(Metallothionein)对镉、铜和锌等多种元素有高度亲和性,具有重金属解毒的功能。选取食用菌中的香菇(Mushrooms)作为代表菌种,在固体培养基中添加了不同浓度梯度的元素锌盐溶液,每天测定不同锌浓度作用下香菇的生长速度;培养结束,测定菌丝体内锌含量和金属硫蛋白含量。结果表明,培养环境中锌浓度越高,菌丝体内富锌量越多。通过一系列元素吸附和金属硫蛋白含量相关性分析,发现元素锌含量与香菇中金属硫蛋白含量呈正相关性,锌含量越高,金属硫蛋白含量也越高。实验结果为香菇及其他食用菌富集元素锌机理提供参考,还为食用菌安全问题的解决提供一定参考依据。

Effect of zinc induction on the growth rate and metallothionein content of shiitake mushroom hyphae

The "three wastes" problem brought about by industrialization has great challenges in fungal food safety. Metallothionein has a high affinity for a variety of heavy metals such as cadmium, copper and zinc, and has the function of detoxifying heavy metals. In this experiment, Mushrooms in edible fungi were selected as representative strains, simulating the pollution of shiitake mushrooms by heavy metals, and heavy metal zinc salt solutions with different concentration gradients were added to the solid medium to determine the growth rate of shiitake mushrooms under different zinc concentrations every day. At the end of the culture, the zinc content and metallothionein content in the mycelium were determined, and the conclusions showed that the higher the zinc concentration in the culture environment, the more zinc rich in the mycelium. The content of heavy metals was positively correlated with the content of metallothionein in shiitake mushrooms, and the higher the zinc-rich content, the higher the metallothionein content. Through a series of heavy metal adsorption and metallothionein content correlation analysis experiments, this paper provides a reference for the research on the mechanism of heavy metal enrichment and MT-induced edible fungus production technology of shiitake mushrooms and other edible fungi, and can also provide a certain reference basis for solving the safety problem of edible fungi, and also prospect the agricultural application prospect of metallothionein.