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Estimated effects of silicone glue on protein crystal growth |
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| Authors: | Mihoko Maruyama Noriko Shimizu Shigeru Sugiyama Yoshinori Takahashi Hiroaki Adachi Kazufumi Takano Satoshi Murakami Tsuyoshi Inoue Hiroyoshi Matsumura Yusuke Mori |
| Affiliation: | 1. Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan;2. SOSHO Inc., Osaka 541-0053, Japan;3. Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan;4. CREST JST, Suita, Osaka 565-0871, Japan |
| Abstract: | Silicone glue (modified silicone polymer) is widely used for both experiments involving inorganic crystal growth and those involving organic materials like proteins. This material is very useful for building a hand-made experiment setup or for fixing protein crystals to specific locations. Though silicone glue is regarded as harmful to proteins, no systematic verification was performed to investigate its impurity effects on protein crystal growth. We focused on and estimated the impurity effects of silicone glue on protein crystal growth. |
| Keywords: | A1. Impurities A1. Optical microscopy A1. Surface processes A2. Growth from solutions B1. Lysozyme B1. Protein |
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