Probing the interaction of a glycoprotein IIb/IIIa receptor antagonist with bound platelets using electrochemical impedance |
| |
| Affiliation: | 1. School of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450000, PR China;2. Department of Chemistry, Tsinghua University, Beijing 100086, PR China;3. Lab of Advanced Power Sources and Key Laboratory of Thermal Management Engineering and Materials, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China;4. National Research Council of Canada, Vancouver, BC, V6T 1W5, Canada;1. School of Chemistry and Materials Science, Ludong University, Yantai 264025, China;2. MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter and Department of Applied Chemistry, School of Science, Xi''an Jiaotong University, Xi''an 710049, China |
| |
| Abstract: | A label-free assay is described to monitor the interaction of abciximab, a glycoprotein IIb/IIIa receptor antagonist (ReoPro), with platelets bound to a fibrinogen-functionalised electrode surface. Firstly, fibrinogen is deposited in a defined pattern onto a gold electrode using microcontact printing, and then platelets from whole blood are captured on the patterned surface. Patterning influences the spreading of platelets, which is strikingly different to that observed on homogeneously coated surfaces. The drug–platelet interaction has been investigated using AC impedance on uniform and patterned fibrinogen-modified surfaces. The results demonstrate that patterned fibrinogen surfaces can provide deep insights into the interaction of abciximab with different platelet sub-populations. The key advantages of this approach are that it is rapid, label free and does not require pre-processing of patient blood samples. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
