首页 | 本学科首页   官方微博 | 高级检索  
     检索      


4-Mercaptophenylboronic acid-induced in situ formation of silver nanoparticle aggregates as labels on an electrode surface
Institution:1. Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, People''s Republic of China;2. Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, Henan 476000, People''s Republic of China;1. Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;2. Analytical Chemistry and Environment Research Unit, Division of Chemistry, Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand;3. Department of Physics, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand;4. Center of Excellence in Nanotechnology for Energy (CENE), Songkhla 90112, Thailand
Abstract:This work presented a general way for in situ formation of citrate-capped silver nanoparticle (AgNP) aggregates as labels on an electrode surface. When the electrode surface was functionalised with a member of the o-diphenol family, 4-mercaptophenylboronic acid (MPBA) was anchored onto the electrode surface via a boronate ester covalent bond. The anchored MPBA captured AgNPs through Agsingle bondS interaction. The resulting surface-tethered AgNPs could recruit more MPBA molecules and AgNPs through the formation of an Agsingle bondS bond and the covalent interaction between the α-hydroxycarboxylate of the citrate and the boronate of the MPBA. This led to in situ formation of a network of AgNPs. The complexes formed between MBPA and citrate acid, as well as dopamine (a member of the o-diphenol family), were characterized by mass spectrometry. The MBPA-induced aggregation of citrate-capped AgNPs in solution was confirmed by UV–Vis spectrophotometry and transmission electron microscopy. The network of AgNPs formed on the diphenol-covered electrode surface was characterized by scanning electron microscopy. The electrochemical signal was measured based on the solid-state Ag/AgCl reaction of the AgNPs. To demonstrate the applications and analytical merits of our design, tyrosinase and protease (thrombin) were measured as model analytes. The proposed strategy is likely to lead to the development of sensors for the detection of other biomolecules.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号