Triangular silver nanoplates: Properties and ultrasensitive detection of miRNA |
| |
| Affiliation: | 1. School of Chemical Sciences, National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland;2. Department of Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, Ireland;3. Department of Bioelectronics, Ecole Nationale Superieure des Mines, CMP-EMSE, MOC, 880 Route de Mimet, Gardanne 13541, France;1. Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Ferrara, via Fossato di Mortara 17, 44121 Ferrara, Italy;2. Dipartimento di Scienze Molecolari e Nanosistemi, Università Ca'' Foscari Venezia, Via Torino 155/B, 30170 Mestre— VE, Italy |
| |
| Abstract: | Triangular silver nanoplates (TSNPs) have been functionalised with probe strand miRNA that is complementary in part to the target nucleic acid, miR-132-3p, that is associated with neuroblastoma. These TSNPs were immobilised in gold microcavities via complementary miRNA hybridisation and can give plasmonic enhancement of the Raman response. Optimum enhancement can be achieved by using different excitation wavelengths and changing the distance between the nanoplate and the surface of the cavity. As silver is electrocatalytically active to the reduction of hydrogen peroxide, these probe-functionalised TSNPs can be used in a miRNA assay where a linear response was obtained for target concentrations from 100 fM to 1 μM target concentration, with a current generated of 100 μA. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
