The conformational features of palytoxin in aqueous solution |
| |
Authors: | Toshiyasu Inuzuka Hirokazu Arimoto |
| |
Institution: | a Graduate School of Life Sciences, Tohoku University, Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan b Department of Biosciences and Informatics, Center for Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan |
| |
Abstract: | Conformational features of palytoxin and acetylated palytoxin were investigated by detailed analyses of NOESY spectra. The conformational differences between palytoxin and acetylated palytoxin may account for the difference in the assembly state of palytoxin, which exists as an associated dimer, and the acetylated derivative, which exists as a monomer in aqueous solution. Two palytoxin units in the dimer may come in contact with each other at the hydrophobic region (C21-40) and the region around two conjugated double bonds (C60-84). The amino group of palytoxin is important for biological activities via Na/K ATPase, but it was not found to be involved in the contact faces of the two palytoxin units. This information should aid in revealing how palytoxin interacts with Na/K ATPase. |
| |
Keywords: | Palytoxin Dimer Na/K ATPase 1H NMR NOESY |
本文献已被 ScienceDirect 等数据库收录! |
|