A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity |
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| Authors: | Umeda Nobuhiro Ueno Tasuku Pohlmeyer Christopher Nagano Tetsuo Inoue Takanari |
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| Affiliation: | Department of Cell Biology, Center for Cell Dynamics, Johns Hopkins University, Baltimore, Maryland 21205, USA. |
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| Abstract: | We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events. |
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