|
|||||
|
|
Designing split reporter proteins for analytical tools |
|
| Authors: | Ozawa Takeaki |
| Institution: | a Department of Molecular Structure, Institute for Molecular Science, 38 Nishigonaka, Myodaiji, Okazaki, Aichi 444-8585, Japan b Department of Chemistry, School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan c Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan |
| Abstract: | A current focus of biological research is to quantify and image cellular processes in living cells and animals. To detect such cellular processes, genetically-encoded reporters have been extensively used. The most common reporters include firefly luciferase, renilla luciferase, green fluorescent protein (GFP) and its variants with various spectral properties. This review describes novel design of split-GFP and luciferase reporters based on protein splicing, and highlights some potential applications with the reporters to study protein-protein interactions, protein localization, intracellular protein dynamics, and protein activity in living cells and animals. |
| Keywords: | Green fluorescent protein Luciferase Protein splicing Protein-protein interaction |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |