Microtiter plate assay for phosphate using a europium-tetracycline complex as a sensitive luminescent probe |
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Authors: | Axel Duerkop Matejka Turel Aleksandra Lobnik |
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Affiliation: | a University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, Universitaetsstrasse 31, D-93040 Regensburg, Germany b University of Maribor, Faculty of Mechanical Engineering, Smetanova 17, SLO-2000 Maribor, Slovenia |
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Abstract: | A new luminescent europium probe is presented for the determination of phosphate (P) in microtiter plate format. The assay is based on the quenching of the luminescence of the europium-tetracycline (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 μmol/L. The probe is excited at 400 nm and displays a large Stokes’ shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7 and is therefore suitable for phosphate determination in biological and biochemical systems. The linear range of the calibration plot is from 5 × 10−6 mol/L to 7.5 × 10−4 mol/L of phosphate, and the limit of detection is 3 μmol/L. |
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Keywords: | Phosphate Microplate assay Luminescence Lanthanide |
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