Reduction of cytochrome c at a lipid bilayer modified electrode |
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| Institution: | 1. Institute of Inorganic Chemistry, RWTH Aachen University, Aachen, Germany;2. Institute of Nanotechnology, Karlsruhe Institute of Technology, Karlsruhe, Germany;3. Rudolf Virchow Center, University of Würzburg, Würzburg, Germany;4. Department of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany;5. Institute for Laboratory Animal Science, Medical Faculty, RWTH Aachen University, Aachen, Germany;1. Fujian Provincial Key Laboratory of Functional Materials and Applications, School of Materials Science and Engineering, Xiamen University of Technology, Xiamen 361024, Fujian, PR China;2. Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan;3. Department of Mechanical, Aerospace, and Biomedical Engineering, University of Tennessee, Knoxville, TN 37996, United States |
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| Abstract: | The reduction of horse heart cytochrome c has been investigated at a platinum electrode modified with a lipid bilayer membrane (BLM) which immobilized vinyl ferrocene as an electron mediator. The current-voltage curves show that the direct electrochemistry of cytochrome c at the metal electrode occurs quite efficiently. An adsorption equilibrium constant for cytochrome at the BLM surface, as well as an electron transfer rate constant between the protein and the modified electrode have been estimated from these results. The values of both parameters are much higher than those reported with other types of electrode modifications, indicating that a lipid bilayer-modified platinum electrode system using vinyl ferrocene as a mediator provides substantial improvements in electrochemical activity of cytochrome c at metal electrodes. The potential for modifying and utilizing this new class of “biomembrane-like” electrode surface for metalloprotein electrochemistry is briefly discussed. |
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