Synthesis of cleavable peptides with authentic C-termini: an application for fully automated SPOT synthesis |
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Authors: | Bernhard Ay |
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Institution: | Institut für Medizinische Immunulogie, Charite—Universitätsmedizin Berlin, Charitéplatz 1, 20-21, 10117 Berlin, Germany |
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Abstract: | A simple method for the synthesis of carboxyl-free peptides on cellulose membranes was improved and adapted for fully automated SPOT synthesis. Using 1,1′-carbonyl-di-imidazole (CDI) or 1,1′-carbonyl-di-(1,2,4-triazole) (CDT) as an activator within a defined period of time, we were able to reduce the formation of di- or oligomerization of the C-terminal amino acid. The soluble peptides are obtained in a purity range of 60-95% and could be used directly for different biological assays (e.g., CD8 T-cell epitope) that require authentic C-termini. |
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Keywords: | CDI CDT SPOT synthesis T-cell epitope mapping |
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