氨基酸/氧化石墨烯/羟基磷灰石复合材料在酸蚀人牙釉质体外再矿化中的应用

本文研究了甘氨酸(Gly)和氧化石墨烯(GO)复合改性羟基磷灰石(Gly/GO/HA),以及天冬氨酸(Asp)和GO复合改性羟基磷灰石(Asp/GO/HA)对酸蚀人牙釉质(HE)体外再矿化的影响。通过CCK-8法测定不同复合材料的细胞毒性,通过场发射扫描电镜、EDX能谱分析、X射线衍射和显微硬度测试,对再矿化前/后HE表面及剖面形貌、表面元素组成、表面成分结构和表面力学性能进行表征。结果表明:Gly/GO/HA和Asp/GO/HA均有良好的安全性和生物相容性,且均能使酸蚀HE得到一定的修复,其中Gly/GO/HA对酸蚀HE表面和深层脱矿的修复效果优于Asp/GO/HA。

Amino Acids/Graphene Oxide/Hydroxyapatite Composites on in vitro Remineralization of Acid-Etched Human Enamel

Effects of glycine (Gly) and graphene oxide (GO) modified hydroxyapatite (Gly/GO/HA), and aspartic acid (Asp) and GO modified hydroxyapatite (Asp/GO/HA) on in vitro remineralization of acid-etched human enamel (HE) were investigated. The cytotoxicity of these composites was studied by CCK-8 method, the surface and cross-section morphology, surface element composition, surface composition structure and surface mechanical properties of HE before and after remineralization were characterized by field emission scanning electron microscope, EDX energy spectrum analysis, X-ray diffraction and microhardness test. The results show that Gly/GO/HA and Asp/GO/HA both have good safety and biocompatibility, and both can repair the acid-etched HE to a certain extent, among which Gly/GO/HA is better than Asp/GO/HA in repairing the surface and deep demineralization of the acid-etched HE.