Gas chromatography-atmospheric pressure chemical ionization-time of flight mass spectrometry for profiling of phenolic compounds in extra virgin olive oil |
| |
Authors: | García-Villalba Rocío Pacchiarotta Tiziana Carrasco-Pancorbo Alegría Segura-Carretero Antonio Fernández-Gutiérrez Alberto Deelder André M Mayboroda Oleg A |
| |
Affiliation: | Department of Analytical Chemistry, Faculty of Sciences, University of Granada, c/ Fuentenueva s/n, E-18071 Granada, Spain. |
| |
Abstract: | A new analytical approach based on gas chromatography coupled to atmospheric pressure chemical ionization-time of flight mass spectrometry was evaluated for its applicability for the analysis of phenolic compounds from extra-virgin olive oil. Both chromatographic and MS parameters were optimized in order to improve the sensitivity and to maximize the number of phenolic compounds detected. We performed a complete analytical validation of the method with respect to its linearity, sensitivity, precision, accuracy and possible matrix effects. The LODs ranged from 0.13 to 1.05ppm for the different tested compounds depending on their properties. The RSDs for repeatability test did not exceed 6.07% and the accuracy ranged from 95.4% to 101.5%. To demonstrate the feasibility of our method for analysis of real samples, we analyzed the extracts of three different commercial extra-virgin olive oils. We have identified unequivocally a number of phenolic compounds and obtained quantitative information for 21 of them. In general, our results show that GC-APCI-TOF MS is a flexible platform which can be considered as an interesting tool for screening, structural assignment and quantitative determination of phenolic compounds from virgin olive oil. |
| |
Keywords: | Gas chromatography Mass spectrometry Atmospheric pressure chemical ionization Phenolic compounds Olive oil |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|