Effect of shear on human insulin in zinc suspension |
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Authors: | Grainger Reid J Ko Samuel Koslov Eugene Prokop Ales Tanner Robert D Loha Veara |
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Institution: | (1) Chemical Engineering Department, Vanderbilt University, 24th and Garland Avenues, 37235 Nashville, TN;(2) Present address: King Mongkut's Institute of Technology, Thonburi, Thailand |
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Abstract: | Human insulin in zinc suspension was used as a model protein to test the effect of shear on the settling rate of proteins,
a possible inference for protein denaturation. The rate of settling was determined directly in a spectropho-tometer. Shear
effects are important in retaining the activity of proteins and are present in bubble, foam, and droplet protein fractionation
processes. A sim pletest, such as that conducted here, mayeven be useful for monitoring changes in protein structure caused
by commercial shipping of the protein. The settling ratefor insulin was continously monitored in theoriginal bottle by spectrophotometric
absorbance changes as a function of time. A settling curve was determined following each shear experiment, which included
shaking the “worked” insulin solution in a vortex mixer for different lengths of time. It was determined, when comparing long
shaking times with short ones, that the initial settling rate was less for the long-term shaking of the insulin samples and
greater for the short-term shaking. The secondary effects of light and heat, along with shaking, a pparently did not produce
differences from shaking alone. |
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Keywords: | Insulin human insulin settling rate shear shear stress shear stress on proteins |
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