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| Effects of Micronutrients on Oxidative Stress and Islet Function in Type 1 Diabetes Mellitus Rats： Relationships to Th2 Type Cytokines, Interleukin-4, and Interluekin-10 | |
| 作者姓名： | ZHANG Gui-zhen LI Mei-hua SONG Yang LIU Ting GAO Shen SUN Ying |
| 作者单位： | [1]Department of Central Research, The Third Clinical College, Jilin University, Changchun 130033, P. R. China; [2]Department of Allergy ＆ Clinical Immunology, Faculty of Medicine, Imperial College School, National Heart ＆ Lung Institute, Dovehouse Street, London SW3 6LY, UK |
| 基金项目： | 国家自然科学基金;吉林省科研项目 |
| 摘 要： | To observe the effects of the micronutrients on oxidative and autoimmune destruction of islets so as to prevent a person from the onset and development of type 1 Diabetes Mellitus(T1DM),the interleukin-4 and interleukin-10 expressions of lymphocytes in peripheral blood and spleen of T1DM rats were determined by flow cytometry.GSH-Px activity and MDA level in the rats' pancreas were measured using biochemical methods.The insulin contents in serum and β cell insulin secret storage were tested by RIA and IHC,respectively.There was an increase in the percentages of IL-4 and IL-10 positive lymphocytes in the peripheral blood and spleen of the groups of rats supplemented with various combinations of micronutrients(p<0.01 and p<0.05,respectively);the blood glucose concentration decreased(p<0.05);both the functional β cell in islets and the insulin content in pancreatic tissue increased(p<0.05 and p<0.01);the GSH-Px activity and MDA level of pancreas in the rats enhanced and decreased respectively(p<0.01 and p<0.05).The results suggest that micronutrients may alleviate the islet lesions by upregulating the expressions of IL-4 and IL-10 and lowering oxidative stress in diabetic rats. |
| 关 键 词： | 糖尿病 微量营养元素 胰岛素 氧压 |
| 文章编号： | 1005-9040（2007）-06-701-04 |
| 收稿时间： | 2007-02-05 |
Effects of Micronutrients on Oxidative Stress and Islet Function in Type 1 Diabetes Mellitus Rats: Relationships to Th2 Type Cytokines, Interleukin-4, and Interluekin-10 |
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| ZHANG Gui-zhen LI Mei-hua SONG Yang LIU Ting GAO Shen SUN Ying.Effects of Micronutrients on Oxidative Stress and Islet Function in Type 1 Diabetes Mellitus Rats: Relationships to Th2 Type Cytokines, Interleukin-4, and Interluekin-10[J].Chemical Research in Chinese University,2007,23(6):701-704. | |
| Authors: | Gui-zhen ZHANG Mei-hua LI Yang SONG Ting LIU Shen GAO Ying SUN |
| Institution: | aDepartment of Central Research, The Third Clinical College, Jilin University, Changchun 130033, P. R. China bDepartment of Allergy & Clinical Immunology, Faculty of Medicine, Imperial College School, National Heart & Lung Institute, Dovehouse Street, London SW3 6LY, UK |
| Abstract: | To observe the effects of the micronutrients on oxidative and autoimmune destruction of islets so as to prevent a person from the onset and development of type 1 Diabetes Mellitus (T1DM), the interleukin-4 and interleukin-10 expressions of lymphocytes in peripheral blood and spleen of T1DM rats were determined by flow cytometry. GSH-Px activity and MDA level in the rats' pancreas were measured using biochemical methods. The insulin contents in serum and β cell insulin secret storage were tested by RIA and IHC, respectively. There was an increase in the percentages of IL-4 and IL-10 positive lymphocytes in the peripheral blood and spleen of the groups of rats supplemented with various combinations of micronutrients (p <0.01 and p <0.05, respectively); the blood glucose concentration decreased (p <0.05); both the functional β cell in islets and the insulin content in pancreatic tissue increased (p <0.05 and p <0.01); the GSH-Px activity and MDA level of pancreas in the rats enhanced and decreased respectively (p <0.01 and p <0.05). The results suggest that micronutrients may alleviate the islet lesions by upregulating the expressions of IL-4 and IL-10 and lowering oxidative stress in diabetic rats. |
| Keywords: | Immunohistochemistry Oxidative stress Micronutrients T1DM Interleukin-4 Interluekin-10 |
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