Correlation between Irradiation Treatment and Metabolite Changes in Bactrocera dorsalis (Diptera: Tephritidae) Larvae Using Solid-Phase Microextraction (SPME) Coupled with Gas Chromatography-Mass Spectrometry (GC-MS) |
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| Authors: | Changyao Shan Baishu Li Li Li Beibei Li YongLin Ren Tao Liu |
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| Affiliation: | 1.Institute of Equipment Technology, Chinese Academy of Inspection and Quarantine, No. A3, Gaobeidianbeilu, Chaoyang District, Beijing 100123, China; (C.S.); (B.L.); (L.L.);2.College of Science, Health, Engineering and Education, Murdoch University, Perth 6150, Australia; |
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| Abstract: | The metabolites produced by the larvae of Bactrocera dorsalis (Diptera: Tephritidae) exposed to different doses of irradiation were analyzed using solid phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS), and a metabonomic analysis method of irradiated insects based on GC-MS was established. The analysis revealed 67 peaks, of which 23 peaks were identified. The metabolites produced by larvae treated with different irradiation doses were compared by multivariate statistical analysis, and eight differential metabolites were selected. Irradiation seriously influenced the fatty acid metabolic pathway in larvae. Using the R platform combined with the method of multivariate statistical analysis, changes to metabolite production under four irradiation doses given to B. dorsalis larvae were described. Differential metabolites of B. dorsalis larvae carried chemical signatures that indicated irradiation dose, and this method is expected to provide a reference for the detection of irradiated insects. |
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| Keywords: | Bactrocera dorsalis (Diptera: Tephritidae) irradiation treatment SPME technology metabolites metabolic pathway |
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