Characterization and Antioxidant Activity of Mannans from Saccharomyces cerevisiae with Different Molecular Weight |
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| Authors: | Yingyuan Zhao Jiaqi Wang Qianzhen Fu Huiru Zhang Jin Liang Wenjie Xue Guoqun Zhao Hiroaki Oda |
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| Affiliation: | 1.College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China; (Y.Z.); (J.W.); (Q.F.); (J.L.);2.Laboratory of Nutritional Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan;3.School of International Education, Henan University of Technology, Zhengzhou 450001, China;4.College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China; |
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| Abstract: | Polysaccharides were extracted from natural sources with various biological activities, which are strongly influenced by their chemical structure and molecular weight. In this research, mannans polysaccharides were obtained from Saccharomyces cerevisiae by ethanol precipitation. The molecular weight of YM50, YM70, and YM90 mannans was 172.90 kDa, 87.09 kDa, and 54.05 kDa, respectively. Scanning electron microscopy of YM 90 mannans showed a rough surface with numerous cavities, while the surfaces of YM50 and YM70 were relatively smooth. Sepharose CL-6B and FTIR indicated that mannans had the characteristic bands of polysaccharides. The antioxidant activities of polysaccharides were evaluated in vitro using various assays. Mannans showed a good scavenging activity of DPPH radicals which depend on the molecular weight and concentration, and a higher scavenging activity of hydroxyl radical than ferric-reducing power activities. For the three types of mannans, cytotoxicity and hemolytic activity were rarely detected in mice erythrocytes and Caco-2 cells. Those results could contribute to the further application of mannans from Saccharomyces cerevisiae in the food and medicine industry. |
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| Keywords: | mannan molecular weight antioxidant activity |
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