甲基红共振瑞利散射法测定多肽药物奥曲肽

采用共振瑞利散射(RRS)法测定奥曲肽,建立了测定痕量奥曲肽的方法。在pH=3.78的B-R缓冲溶液中,奥曲肽与甲基红相互作用后,共振瑞利散射显著增强,在280～370nm范围内呈现高的散射强度,且以310nm处的ΔIRRS最强。在优化的测试条件下,奥曲肽浓度在0～0.54mg/L范围内与散射强度有良好的线性关系,对奥曲肽的检出限(3σ)达3.6μg/L。该方法用于合成样品中奥曲肽的测定,回收率为98.3%～100.7%;用于奥曲肽注射液中奥曲肽含量的测定,结果与HPLC法测得的结果相近。

Determination of Octreotide by Resonance Rayleigh Scattering Method with Methyl Red

An ultrasensitive resonance rayleigh scattering(RRS) method for the determination of trace octreotide was developed. The determination of octreotide in an octreotide methyl red system via RRS was studied comprehensively. In a pH=3.78 B-R buffer solution, the RRS intensity is enhanced obviously in the range of 280~370 nm as octreotide associates with methyl red. The strongest ΔIRRS is at 310 nm. The RRS intensitiy of octreotide is proportional to the concentration of octreotide over the range of 0~0.54 mg/L, the detection limit for the octreotide(3σ) is 3.6 μg/L under optimum conditions. The method was applied to the determination of octreotide in synthetic samples with the recovery of 98.3%~100.7%. Measurements of the commercial injection samples also gave satisfactory results which were in good agreement with those obtained using high performance liquid chromatography(HPLC) method.