High-Performance Liquid Chromatography with Electrospray Mass Spectrometry for Rapid and Sensitive Determination of Sanguinarine and Chelerythrine in Exogenously Contaminated Honey |
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Authors: | Xu-biao Luo Bo Chen Shou-zhuo Yao |
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Institution: | (1) State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, P.R. China;(2) Chemical Research Institute, Hunan Normal University, Changsha, 410081, P.R. China |
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Abstract: | A simple, rapid, and sensitive high-performance liquid chromatographic (HPLC) method coupled with electrospray mass spectrometry (ESI-MS) has been used to determine sanguinarine and chelerythrine in exogenously contaminated honey. Sample extracts were separated on a C8 reversed-phase HPLC column with acetonitrile–acetate buffer (40:60) as mobile phase. After ESI the abundance of protonated molecules was recorded by selected-ion recording (SIR) of m/z 332.5, 348.5, and 356.5 for sanguinarine, chelerythrine, and the internal standard, tetrahydropalmatine, respectively. The internal standard technique was used to construct calibration plots for quantitation of sanguinarine and chelerythrine; the linear ranges were 5.25–1050 and 3.75–750 ng mL–1, respectively, with correlation coefficients of 0.9993 and 0.9989, respectively. The limits of detection for sanguinarine and chelerythrine were 1.60 and 1.11 ng mL–1, respectively. |
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Keywords: | Column liquid chromatography-mass spectrometry Sanguinarine and Chelerythrine Tetrahydropalmatine internal standard Honey |
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