Spectrophotometric determination of ofloxacin in pharmaceuticals by redox reaction |
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Authors: | P J Ramesh K Basavaiah N Rajendraprasad O Zenita Devi K B Vinay |
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Institution: | (1) Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt;(2) Department of analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt |
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Abstract: | Two simple spectrophotometric methods have been developed to analyze ofloxacin (OFX) in pharmaceuticals. The methods are based
on the oxidation of OFX by a measured excess of cerium(IV) sulfate in H2SO4 medium. This was followed by the determination of the unreacted oxidant by reacting it with either p-toluidine (p-TD) and measuring the absorbance at 525 nm (method A) or o-dianisidine (o-DA) and measuring the absorbance at 470 nm (method B). In both methods, the amount of cerium(IV) sulfate reacted corresponds
to the amount of OFX. Calibration graphs were linear over the ranges of 0–120 and 0–4 g/ml OFX for methods A and B, respectively.
The calculated molar absorptivity (2.34⋅103 and 5.99⋅104), Sandell sensitivity, and limit of quantification for the methods are reported. The intra-day precision (%RSD) and accuracy
(%RE) were < 8.0 and ≤ 4.0%, respectively, and the inter-day RSD and RE values were within 5 and 4.0%, respectively. The applicability
of the methods was demonstrated by determining OFX in tablets with an accuracy (%RE) of < 3% and precision (%RSD) of ≤2.65%.
The accuracy of the methods was further ascertained by recovery experiments via a standard-addition procedure. |
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