| 抗牛免疫缺陷病毒（BIV）穿膜蛋白gp45杂交瘤细胞株的建立 |
| |
| 引用本文： | 王岱,黄建英.抗牛免疫缺陷病毒（BIV）穿膜蛋白gp45杂交瘤细胞株的建立[J].南开大学学报,1996,29(1):59-63. |
| |
| 作者姓名： | 王岱 黄建英 |
| |
| 作者单位： | 南开大学生命科学学院，天津市肿瘤研究所 |
| |
| 基金项目： | 国家教委优秀年轻教师基金,天津市自然科学基金 |
| |
| 摘 要： | 编码牛免疫缺陷病毒（ＢＩＶ）穿膜蛋白的一个４００ｂｐ的基因片断被克隆到ｐＡＴＨ表达载体上，此 重组的融合蛋白ＴｒｐＥ－Ｅｎｖ８在大肠杆菌中得到高效表达，而且包含有与ＢＩＶ抗体特异性反应的抗原决定簇。我们用此融合蛋白免疫ｂａｌｂ／ｃ小鼠得到与ＢＩＶ穿膜蛋白特异性反应的杂交瘤。此单克隆抗体在蛋白印迹法分析中与重组的ＴｒｐＥ－Ｅｎｖ８有特异性反应，用免疫酶染色法可检测ＢＩＶ在体外的复制。
|
| 关 键 词： | 牛 免疫缺陷病毒 穿膜蛋白 单克隆抗体 杂交瘤 |
GENERATION OF A HYBRIDOMA REACTIVE AGAINST BOVINE IMMUNODEFICIENCY VIRUS TRANSMEMBRANE PROTEIN |
| |
| Wang Dai, Wei Xin, Liu Shuhong, Geng Yunqi.GENERATION OF A HYBRIDOMA REACTIVE AGAINST BOVINE IMMUNODEFICIENCY VIRUS TRANSMEMBRANE PROTEIN[J].Acta Scientiarum Naturalium University Nankaiensis,1996,29(1):59-63. |
| |
| Authors: | Wang Dai Wei Xin Liu Shuhong Geng Yunqi |
| |
| Abstract: | A 400bp gene segment encoding a partial peptide of Bovine Immunodeficiency Virus (BIV)transmembrane protein was cloned into pATH expression vector. The recombinant fusion protein trpE-Env8 expressed at a high level in E. colt and containing a major immunoreactive epitope. The fusion protein was used to immunize balb/c mice to generate hybridoma specifically against BIV transmembrane protein. The Mab was specific for the TrpE-Env8 protein in western blotting assay and detected specifically the BIV replication in vitro by immunoperoxidase assay. |
| |
| Keywords: | Bovine Immunodeficiency Virus (BIV) transmembrane protein gp45 monoclonal antibody |
| 本文献已被 CNKI 维普 等数据库收录！ |
