Analysis of tissue glycoprotein sugar chains by two-dimensional high-performance liquid chromatographic mapping |
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| Authors: | S Hase K Ikenaka K Mikoshiba T Ikenaka |
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| Affiliation: | Department of Chemistry, Osaka University, College of Science, Japan. |
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| Abstract: | Excellent separation of 45 pyridylamino derivatives of oligosaccharides were achieved by the two-dimensional combination of reversed-phase and size-fractionation high-performance liquid chromatography. The sugar chains of brain glycoproteins were derivatized into a mixture of pyridylamino-oligosaccharides from lyophilized brain tissue without any purification steps, and they were well separated by the system used. The pattern obtained was reproducible, and inter-individual variation was negligible. This finding demonstrated the possibility that this method could be applied to the detection of differences in the structure of glycoprotein sugar chains in crude preparations. |
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