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胰蛋白酶溶液的激光拉曼谱
引用本文:柯惟中,余多慰.胰蛋白酶溶液的激光拉曼谱[J].光散射学报,1996,8(3):142-146.
作者姓名:柯惟中  余多慰
作者单位:南京师范大学理化中心
摘    要:本文报道了用硝基苯萃取处理前后的胰蛋白酶溶液的拉曼光谱。通过分析酰胺Ⅰ和酰胺Ⅲ的拉曼特征频率,初步推断出该胰蛋白酶是含有α-螺旋、β-折叠和无规卷曲结构。同时对其侧链结构也做了初步分析

关 键 词:拉曼光谱  胰蛋白酶  硝基苯

Raman Spectra of Trypsin in Aqueous Solution
Ke Weizhong and Yu Duowei.Raman Spectra of Trypsin in Aqueous Solution[J].Chinese Journal of Light Scattering,1996,8(3):142-146.
Authors:Ke Weizhong and Yu Duowei
Abstract:The Raman spectra of native trypsin in aqueous solution and trypsin extracted by nitrobenzene have been obtained. From a combined analysis of the amide Ⅰ and amide Ⅲ bands it is concluded that trypsin consists of α helix, β sheet and random coil. The side chain conformation of trypsin also has been studied.
Keywords:Raman spectroscopy  trypsin  nitrobenzene
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