| STUDIES ON TRANSPLANTATION OF THE LAMININ RECEPTOR AND ITS ROLES IN EXPERIMENTAL METASTASIS OF MURINE LEWIS LUNG CARCINOMA CELLS |
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| 引用本文： | 张青云,周柔丽. STUDIES ON TRANSPLANTATION OF THE LAMININ RECEPTOR AND ITS ROLES IN EXPERIMENTAL METASTASIS OF MURINE LEWIS LUNG CARCINOMA CELLS[J]. 中国科学B辑(英文版), 1992, 0(8) |
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| 作者姓名： | 张青云 周柔丽 |
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| 作者单位： | Beijing Institute for Cancer Research，Department of Cell Biology，Beijing Medical Universtry，Beijing 100083，PRC |
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| 基金项目： | Project supported by the National Natural Science Foundation of China. |
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| 摘 要： | The isolated laminin receptor (LN-R) labeled by ~(125)I was reconstituted into liposomes.~(125)I-LN-R-liposomes and free ~(125)I-LN-R were separated by Sepharose 4B column chromatogra-phy. The LN-R-liposomes showed affinity for laminin (LN) and were capable of binding toimmobilized LN substrate. In order to make transplantation of LN-R, LN-R-liposomes werefused with cultured murine Lewis lung carcinoma cells with the help of polyethylene glycol(PEG) induction. The radiation with the fused cells was not removed by salt sclution. Thebinding of the fused cells enriched in foreign LN-R to LN substrate increased by 87.5%.Furthermore, the murine Lewis lung carcinoma cells with and without transplanted LN-Rwere injected into C_(57)BL/6J mice through tail veins (5×10~5 cells/each mouse)respectively.The mice in the test group died earlier than those in the control group. The total weight oflung tumor in the test group remarkably increased in comparison with those in the controlgroup. The results taken together
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STUDIES ON TRANSPLANTATION OF THE LAMININ RECEPTOR AND ITS ROLES IN EXPERIMENTAL METASTASIS OF MURINE LEWIS LUNG CARCINOMA CELLS |
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| ZHANG QING-YUN,ZHOU ROU-LI. STUDIES ON TRANSPLANTATION OF THE LAMININ RECEPTOR AND ITS ROLES IN EXPERIMENTAL METASTASIS OF MURINE LEWIS LUNG CARCINOMA CELLS[J]. Science in China(Chemistry), 1992, 0(8) |
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| Authors: | ZHANG QING-YUN ZHOU ROU-LI |
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| Affiliation: | ZHANG QING-YUN,ZHOU ROU-LI Department of Cell Biology,Beijing Medical Universtry,Beijing 100083,PRC Present address: Beijing Institute for Cancer Research. |
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| Abstract: | |
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| Keywords: | laminin receptor liposome receptor transplantation Lewis lung carcinoma cancer metastasis |
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