Intensification of the Emission Signals Using the Reflection Mirrors Mounted to the Sample Holder of a Fluorimeter

Effects of the reflection mirrors mounted to the sample holder of a fluorimeter have been investigated. Analytical expressions are given for the emission intensity measured as a function of the concentration or optical density of the sample, in case one or two reflection mirrors are attached beside the sample holder to intensify the emission signals. The emission intensity calculated as a function of the sample concentration agreed well with the experimental data. By mounting two reflection mirrors, the emission signal was intensified by a factor of near 3.5. However, the degree of intensification depended strongly on the sample concentration and the linearity between the sample concentration and the emission intensity deviated significantly with increasing the sample concentration.