A METHOD FOR THE ELECTROCHEMICAL INITIATION OF THE in vitro BACTERIAL LUCIFERASE REACTION |
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| Authors: | Hajime Karatani Takahiro Shizuki Rozana Halon Eiichiro Nakayama |
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| Affiliation: | Department of Polymer Science and Engineering, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606, Japan;Research Center for Instrumental Analysis, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan |
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| Abstract: | Abstract— We have developed an electrochemical initiation method for the in vitro bacterial luciferase reaction using Vibrio harveyi luciferase. The cathodic conversion of oxidized riboflavin 5'-phosphate (FMN) to its reduced form (FMNH2), on which the kinetics for the overall light-emitting reaction depend, is followed by the bacterial luciferase reaction. The electrochemical initiation method can be used for both turnover and nonturnover luciferase reactions, depending on the FMN electroreduction methods. |
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