免疫组化方法检测猪和人皮肤通用抗原标志

目的　探讨猪与人皮肤组织蛋白抗原性的差异。方法　人和小猪全厚正常皮肤作冰冻切片 ,用抗人皮肤组织相关抗原 (HLA ABC ,HLA DR ,Vimentin ,Desmin ,Actin ,FⅧRA ,Cytokeratin)、细胞因子及其受体和胞外基质 (Hyalu ronicacid ,Fibronectin ,Laminin ,TypeⅣcollagen ,Estradiol 17β和Testosterone)及Ⅰ Ⅲ Ⅶ型胶原等抗原成分的单克隆或多克隆抗体进行免疫组化染色 ,比较猪与人皮肤组织抗原性异同。结果　在猪皮肤组织可检测到I Ⅲ Ⅳ Ⅶ型胶原和Hyaluronicacid、Fibronectin、Laminin、FⅧRA、Cytokeratin、IL 6、IL 8、Estradiol 17β、Testosterone及ICAM 1;多克隆抗体阳检率明显高于单克隆抗体 ;其中细胞外基质成分和分子量较小的细胞因子 ,以及结构或功能原始的蛋白分子在猪与人皮肤有较好的同源性 ,而某些蛋白分子 (如细胞因子或激素的受体和淋巴细胞CD分子等 )则多为阴性 ,显示抗体 抗原结合反应的特异性及其种族差异。结论　猪与人皮肤组织抗原之间存在很大的差异 ,猪血管内皮细胞表达人ICAM 1,提示二者粘附分子可能存在物种间的交叉 ,可能与异种皮肤移植难以存活有关。本研究为异种脱细胞真皮基质的临床应用提供了有用的资料

Determination of Skin Common Antigen Marker of Pig and Human with Immunohistochemical Method

Objective\ To investigate the difference of antigenicity between human and pig skin.Methods\ Frozen se ction of full thickencss skin of human and pig were stained with immunohistochemical method,using the following anti\|human antibodies:HLA\|ABC,HLA\|DR,vimentin,desmin,actin,FⅧRA,cytokeratin,hyaluronic acid,fibronectin,laminin,type Ⅰ/Ⅲ/Ⅳ and Ⅶ collagen,estradiol\|17β,and testosterone.Results\ The antigens of collagen of types Ⅰ,Ⅲ,Ⅳ,Ⅶ,hyaluronic acid,fibronectin,laminin,FⅧRA,cytokeratin,IL\|6,IL\|8,estradiol\|17β,testosteron,and ICAM\|1 were detected in pig skin.The positive rate was higher with polyclonal antibodies than with in monoclonal antibodies.There are relatively good cross reactivity of extracellular matrix(ECM),cytokins with smaller molecules,and proteins with primitive functions,such as IL\|6,IL\|8,ICAM\|1,estradiol\|17β and testosterone in skin of human and pig. However,the cross reactivity of recipents of cytokins and CD(cluster of differentiation)molecule in skin were relatively poor.The results showed the specificity and species variation of antigen and antibodies.Conclusion\ Antigens are different between human skin and pig skin.This may be the possible reason for rapid rejection following skin xeno grafting.